Products

HBV BSC-cccDNA PCR Quantification Kit v55

  • Standardized assay with reproducible results
  • Suitable for HBV genotypes A-D
  • Suitable for genomic and fragmented DNA
  • Specific and sensitive for up to 100 copies (linear range 102 – 106 copies)
  • Amplicon size is 55bp
  • For research use only

The HBV cccDNA PCR Quantification kit is a real-time PCR assay for the quantification of bisulfite-converted (BS) hepatitis B virus (HBV) covalently closed circular DNA (cccDNA). The kit provides reagents optimized for rapid and sensitive detection using the Roche LightCycler® 480.

Product Details

The PCR kit is intended to quantify HBV cccDNA after bisulfite conversion. The kit utilizes SYBR Green dye for quantification on Roche LightCycler® 480. Reagents in kit includes assay mix and  standard DNA template. There are sufficient amount of reagents for 50 reactions.

The kit does not contain LightCycler® 480 SYBR Green I Master or bisulfite conversion reagents.

Pricing

Product Name (Cat #)DescriptionUnit Price
HBV BSC-cccDNA Quantification Kit v55 (qPCR-021-v55)One kit for 50 reactions$660

Figure 1: Typical amplification and standard curve generated by Roche LightCycler® 480. Assay shows sensitivity to 10 copies of positive control DNA and linearity from 100 to 103 copies.