Products

HBV cccDNA Quantification Kit

  • Standardized assay with reproducible results
  • Suitable for HBV genotypes A-D
  • Suitable for genomic and fragmented DNA
  • Ready to use system with simple workflow
  • Specific and sensitive for up to 100 copies
  • For research use only

The HBV cccDNA quantification kit contains reagents for bisulfite (BS) DNA conversion and real-time qPCR assay for the quantification of BS-converted hepatitis B virus (HBV) covalently closed circular DNA (cccDNA). The kit is optimized for rapid and sensitive detection using the Roche LightCycler® 480.

Product Details

The kit is intended to quantify HBV cccDNA after bisulfite conversion. The kit utilizes BS DNA conversion and SYBR green-based qPCR for quantification on Roche LightCycler® 480. Reagents in the kit includes BS DNA conversion and clean-up, assay mix and standard and background control DNA templates. There are sufficient amount of reagents for 50 reactions.

The kit does not contain LightCycler® 480 SYBR Green I Master.

Pricing

Product Name (Cat #)DescriptionUnit Price
HBV cccDNA Quantification Kit (JBS-08878-021)One kit for 50 reactions$800

Figure 1: Typical amplification and standard curve generated by Roche LightCycler® 480. Assay shows sensitivity to 100 copies of positive control DNA and linearity from 100 to 103 copies.