Service

Targeted NGS of HCC-associated genes

We offer NGS-based variant detection in 43 HCC-linked genes and intergenic regions. Both somatic and germline variants can be identified. Key features of the service include:

  • Construction and QC of UMI libraries from cfDNA or sheared genomic DNA.
  •  Hybridization enrichment using two different capture probe panels, A and B (shown below).
  • High (60-80%) on-target capture rates and excellent coverage uniformity, with over 99% of all target positions covered within a twofold difference of the mean. 
  • Sequencing at a depth required for detection of variants at specified somatic frequencies.
  • Bioinformatic analyses including UMI-based read consolidation with a specified family size and variant detection.

For questions and pricing

Please send inquiries to info@jbs-science.com

Panel A
ALB
APC
APOB
ARID1A
ARID1B
ARID2
ATM
AXIN1
BAP1
CDKN2A
CTNNB1
HNF1A
IL6ST
KEAP1
KMT2D
LRP1B
NFE2L2
PCLO
PIK3CA
RB1
RPS6KA3
TERT promoter
TP53
TSC2

Panel B

ABCC13

CCND1

CCNE1

CMYC

CMYC-PVT1 intergenic

DNAH8

EEF1A1

ERRFI1

FN1

IDH1

IDH2

LZTR1

MCL1

MET

NCOR1

PTEN

SMARCA4

TERT

VEGFA

WWP1