Duplex RASSF1A and GSTP1 Methylation Quantification Kit
- Standardized assay with reproducible results
- Ready to use system with simple workflow
- Specific and sensitive for up to 3 copies of methylated RASSF1A and GSTP1 DNA
- For research use only
The kit is a duplex real-time PCR assay for detecting methylated RASSF1A promoter DNA targeting the P1 region and methylated GSTP1 promoter DNA. The kit provides reagents optimized for rapid and sensitive detection using the Roche LightCycler® 480.
The PCR kit is intended to quantify methylated RASSF1A DNA of 52bp in size and methylated GSTP1 DNA of 46bp in size. The kit utilizes Naica Multiplex PCR Mix, 5x for quantification on the Roche LightCycler® 480. Reagents in the kit include assay mix, standard DNA control, and background DNA control. There are sufficient amount of reagents for 50 reactions.
The kit does not contain Naica Multiplex PCR Mix 5x (Stilla Technologies).
|Product Name (Cat #)
|Duplex RASSF1a and GSTP1 Methylation Quantification Kit (qPCR-019)
|One kit for 50 reactions
Figure 1. Typical amplification and standard curve generated by Roche LightCycler® 480. Assay shows sensitivity to 3 copies of positive control DNA and linearity from 10 to 104 copies.