SEPT9 Short Amplicon Methylation specific qPCR kit
- Standardized assay with reproducible results
- Ready to use system with simple workflow
- Specific and sensitive up to 10 copies of methylated DNA
- For research use only
The SEPT9 Short Amplicon Methylation specific qPCR kit is a one-step, real-time PCR assay for the quantification of the methylated SEPT9. The kit provides reagents optimized for rapid and sensitive detection using the LightCycler® 480 instrument (Roche).
The SEPT9 Short Amplicon Methylation specific qPCR kit is intended to quantify the methylated SEPT9. The kit utilizes a once-step approach (LightCycler® 480 SYBR Green Master I) for quantification of PCR products on Roche’s LightCycler® 480 platform. Reagents in the kit include primers, and controls sufficient for 50 reactions.
The kit does not contain LightCycler® 480 SYBR Green Master I.
|Product Name (Cat #)
|SEPT9 Short Amplicon Methylation PCR Quantification Kit (qPCR-006)
|One kit for 50 reactions
Figure 1: Typical amplification curve generated by Roche’s LightCycler® 480. Assay shows sensitivity to 10cp and linearity from 103cp to 10cp of Hypermethylated SEPT9.