RASSF1A Short Amplicon Methylation specific qPCR kit
- Standardized assay with reproducible results
- Ready to use system with simple workflow
- Specific and sensitive up to 10 copies of methylated DNA
- For research use only
The RASSF1A Short Amplicon Methylation specific qPCR kit is a two-step, real-time PCR assay for detecting methylated RASSF1A promoter targeting the P1 region. The kit provides reagents optimized for rapid and sensitive detection using the HotStarTaq Polymerase (Qiagen) and the LightCycler® 480 instrument (Roche).
The RASSF1A Short Amplicon Methylation specific qPCR kit is intended to quantify the methylated RASSF1A promoter. The kit utilizes a two-step approach (HotStart Taq Plus and LightCycler® 480 SYBR Green Master I) for quantification of PCR products on Roche’s LightCycler® 480 platform. Reagents in the kit include first and second step primers and controls sufficient for 50 reactions.
The kit does not contain LightCycler® 480 SYBR Green Master I.
|Product Name (Cat #)
|RASSF1A Short Amplicon Methylation PCR Quantification Kit (qPCR-004)
|One kit for 50 reactions
Figure 1: Typical amplification curve generated by Roche’s LightCycler® 480. Assay shows sensitivity to 10cp and linearity from 1250cp to 10cp of methylated RASSF1A.