CTNNB1 Duplex T41A and S45F Droplet Digital PCR Quantification Kit

Products

CTNNB1 Duplex T41A and S45F Droplet Digital PCR Quantification Kit

Standardized assay with reproducible results
Simple workflow
Specific and sensitive up to 0.1% of mutated CTNNB1 DNA at codons 41 (p.T41A) and 45 (p.S45F)
For research use only

The CTNNB1 duplex T41A and S45F droplet digital PCR (ddPCR) detects mutations located at codon 41 (hg38, Chr3:41224633A>G) and codon 45 (hg38, Chr3: 41224646C>T). The kit provides assay mix and DNA standard controls for highly sensitive mutation detection down to 0.1% (Figure 1). The kit was developed on the Naica^® system (Stilla Technologies) ddPCR platform. The kit does not contain mastermix buffer.

Pricing

Product Name (Cat #)	Description	Unit Price
CTNNB1 Duplex T41A and S45F Droplet Digital PCR Quantification Kit (ddPCR-003)	One kit for 50 reactions	$950

Figure 1: Assay performance. The duplex T41A and S45F ddPCR assay contains probes targeting T41A mutant DNA (FAM), S45F mutant DNA (Cy5) and wild-type DNA at codon 41 (HEX). DNA standards reconstituted at 100%, 10%, 1%, and 0.1% are shown for the T41A mutation and S45F mutation in a background of 3,000 copies of wild-type DNA. No mutant droplets were detected in negative control samples. WT, wild-type DNA of 5,000 copies; NTC, no-template-control.