Products
JBS Bisulfite Conversion (BSC) Assay Kit
- Standardized assay with reproducible results
- Ready to use system with simple workflow
- Specific and sensitive for up to 10 copies
- For research use only
The JBS Bisulfite Conversion (BSC) assay kit is a real-time PCR assay for the evaluation of bisulfite conversion. The kit provides reagents optimized for rapid and sensitive detection using the Roche LightCycler® 480 or LightCycler® 96 instrument
Product Details
JBS BSC assay kit is a methylation-independent qPCR assay that targets a 64 bp region on human chromosome 15. The bisulfite-converted DNA qPCR product has a more distinct melting peak than the corresponding untreated DNA. Thus, by comparing the same DNA that has been BSC-treated and non-treated, one can evaluate the performance of the bisulfite conversion kit used. The kit utilizes SYBR Green dye for quantification on Roche LightCycler® 480 or LightCycler® 96 platform. Reagents in kit include assay mix and assay DNA. There are sufficient amount of reagents for 50 reactions
The kit does not contain LightCycler® 480 SYBR Green I Master.
Pricing
| Product Name (Cat #) | Description | Unit Price |
| Bifulfite conversion (BSC) assay Kit (qPCR-030) | One kit for 50 reactions | $550 |
Red: bisulfited treated DNA; Green: same DNA not treated
| Reference | ||
| Standards | Cq | Tm |
| NTC | – | – |
| sHug (0.02 ng or 6.06 copies) | 34-36 | 74 |
| sHug (0.2 ng or 60.6 copies) | 31 | 74 |
| sHug (2 ng or 606 copies) | 27-28 | 74-75 |
| sHug (20 ng or 6,060 copies) | 24 | 75 |
Typical amplification and standard curve generated by Roche LightCycler® 480. Assay shows sensitivity to 10 copies of DNA.