muBRAF V600E PCR Quantitation Kit
The muBRAF V600E PCR Kit is a two-step, real-time PCR assay for the quantification of mutated BRAF V600E DNA. The kit provides reagents optimized for rapid and sensitive detection using HotStarTaq Polymerase (Qiagen) and the Roche LC480 instrument.Product Details
The muBRAF V600E PCR kit is intended to quantify mutated (V600E) BRAF sequences. The kit utilizes a two-step approach (HotStarTaq and LC480 SYBRGreen) for quantification of PCR products on Roche Light Cycler 480 platform. Reagents in the kit include first and second step primers, positive and negative control DNA sufficient for 50 reactions.
The kit does not contain Light Cycler 480 SYBRGreen.Pricing
Kit Price: 50 reactions for $950.00
Figure 1: Typical amplification curve generated by Roche Light Cycler 480. Assay shows sensitivity to 10cp and linearity from 104cp to 100cp of muBRAF V600E in positive control cell line DNA in a background of 1ng negative control cell line.