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CTNNB1 32-37 mutation assay
  • Standardized assay with reproducible results
  • Ready to use system with simple workflow
  • Specific and sensitive up to 10 copies of mutated CTNNB1 (codons 32-37) DNA
  • For research use only

The CTNNB1 32-37 Mutation Analysis Kit is a two-step, real-time PCR assay for the quantification of mutated CTNNB1 (codons 32-37) DNA. The kit provides the DNA oligos, standards, and protocol - standard PCR reagents are needed for rapid and sensitive detection using this kit. The kit utilizes a two-step approach (HotStarTaq and hydrolysis probe) for quantification of PCR products on a Roche Light Cycler 480 platform. The kit does not contain enzymes, reagents, or buffers.

Pricing

Kit Price: 50 reactions for $950.00

Background

The human CTNNB1 gene encodes Catenin beta-1 (or Beta-catenin), which has important functions in cell-cell adhesion and gene transcription. Beta-catenin is part of the Wnt signaling pathway, where it acts as a subunit of the cadherin protein complex. Mutations in CTNNB1 that affect overexpression of Beta-catenin are largely observed in cancers such as hepatocellular carcinoma, colorectal carcinoma, lung cancer, malignant breast tumors, ovarian and malignant endometrial cancer.

Nearly 10% of all cancers are found to contain CTNNB1 mutations. Cancer causing mutations typically affect the N-terminal segment of Beta-catenin, which prevents degradation and ultimately results in Beta-catenin translocating to the nucleus to drive transcription of target genes. While there are several mutations reported, they are mostly localized between codons 32-45. This kit is capable of detecting mutations in codons 32-37, which make up nearly half of all CTNNB1 mutations in cancer.


CTNNB1 32-37 assay manual
muBRAF

Figure 1: Typical amplification curve generated by Roche Light Cycler 480. Assay shows sensitivity to 10cp and linearity from 10,000cp to 10cp of a CTNNB1 mutant plasmid (S37C mutation) in a background of 1ng human genomic cell line. Note, any mutation in codons 32-37 will be detected by this assay.


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